mouse testicular sertoli cell line tm4  (ATCC)

Journal: Toxics

Article Title: PFOA Damages Blood–Testis Barrier Integrity in Mice by Inhibited Glycolysis Caused H3K18 Lactylation Modification Impairment

doi: 10.3390/toxics14050399

Figure Lengend Snippet: PFOA altered the expression of glycolysis-related genes. Based on RNA-seq analysis, the total RNA expression profiles of testicular tissues in the control and 5 mg/kg/day PFOA-exposed groups were compared ( A ). KEGG pathway enrichment ( B ) and GO functional enrichment analyses ( C ) of differentially expressed genes revealed that the glycolysis pathway served as a key target of PFOA. After 24 h of PFOA treatment in TM4 cells, cell viability was decreased ( D ), accompanied by significant changes in the expression of glycolysis-related genes ( E ). ( n = 3, * p < 0.05; ** p < 0.01; *** p < 0.001, * vs. control group).

Article Snippet: This experiment utilized a mouse testicular sertoli cell line (TM4) obtained from the ATCC cell bank in the United States.

Techniques: Expressing, RNA Sequencing, RNA Expression, Control, Functional Assay

Journal: Toxics

Article Title: PFOA Damages Blood–Testis Barrier Integrity in Mice by Inhibited Glycolysis Caused H3K18 Lactylation Modification Impairment

doi: 10.3390/toxics14050399

Figure Lengend Snippet: PFOA inhibited the expression of glycolysis and BTB-related molecules in TM4 cells and mouse testicular tissues. In PFOA-treated TM4 cells, the protein levels of key glycolytic molecules, including HK1 ( B ), GLUT1 ( C ) and LDHA ( D ), were downregulated ( A – D ), accompanied by reduced production of lactate ( E ) and ATP ( F ). Meanwhile, both the gene ( G ) and protein ( H ) expression of BTB-related factors were decreased in TM4 cells. Consistent with the cellular results, the mRNA ( I ) and protein ( J ) levels of glycolysis-related molecules were also suppressed in mouse testis, along with a decline in lactate concentration ( K ). ( n = 3, * p < 0.05; ** p < 0.01; *** p < 0.001, * vs. control group).

Article Snippet: This experiment utilized a mouse testicular sertoli cell line (TM4) obtained from the ATCC cell bank in the United States.

Techniques: Expressing, Concentration Assay, Control

Journal: Toxics

Article Title: PFOA Damages Blood–Testis Barrier Integrity in Mice by Inhibited Glycolysis Caused H3K18 Lactylation Modification Impairment

doi: 10.3390/toxics14050399

Figure Lengend Snippet: Effects of PFOA on H3K18 lactylation and the transcriptional regulation of key genes involved in glycolysis. PFOA exposure reduced the modification levels of Pan-Kla and H3K18la in TM4 cells ( A ) and mouse testicular tissues ( B ). ChIP-qPCR results further demonstrated that PFOA altered the binding of H3K18la to glycolytic genes, thereby regulating their transcription ( C ). ( n = 3, * p < 0.05; ** p < 0.01; *** p < 0.001, * vs. control group).

Article Snippet: This experiment utilized a mouse testicular sertoli cell line (TM4) obtained from the ATCC cell bank in the United States.

Techniques: Modification, ChIP-qPCR, Binding Assay, Control

Journal: Toxics

Article Title: PFOA Damages Blood–Testis Barrier Integrity in Mice by Inhibited Glycolysis Caused H3K18 Lactylation Modification Impairment

doi: 10.3390/toxics14050399

Figure Lengend Snippet: Protective effects of Nala against PFOA-induced reproductive damage. Gradient Nala treatment for 24 h significantly elevated the levels of Pan-Kla and H3K18la in TM4 cells ( A ). After co-treatment with 30 mM Nala and 400 μM PFOA for 24 h, the PFOA-mediated reductions in Pan-Kla and H3K18la were notably restored ( B ). The decreased expression of Hk1, Glut1 and Ldha was partially rescued ( C ), and the downregulated mRNA ( D ) and protein ( E ) levels of BTB-related molecules were also markedly recovered. ( n = 3, * p < 0.05; ** p < 0.01; *** p < 0.001; * vs. control group. # p < 0.05; ## p < 0.01; ### p < 0.001; # vs. PFOA group).

Article Snippet: This experiment utilized a mouse testicular sertoli cell line (TM4) obtained from the ATCC cell bank in the United States.

Techniques: Expressing, Control

Journal: Cell Biology and Toxicology

Article Title: ATG5-dependent autophagy in Sertoli cells protects against cadmium-disrupted blood-testis barrier via perturbing CXCL2/CXCR2 axis

doi: 10.1007/s10565-025-10089-5

Figure Lengend Snippet: Cd exposure activates autophagy in Sertoli cells. A - G The male mice were injected (i.p.) with 2.0 mg/kg CdCl 2 or vehicle, respectively. Mouse testes were collected at 0, 2, 8, 24, 48, and 72 h after Cd treatment. A , B Representative images and quantification of autophagosomes in mice Sertoli cells detected by transmission electron microscopy. The yellow arrows represent the autophagosomes. C - E The expression of ATG5 and LC3B-II/I was detected by western blotting. F , G Representative images and quantification of mIHC assay. White arrows denoted the LC3B puncta surrounding WT1. Scale bar: 50 μm. H - J TM4 cells were exposed to Cd for 0, 2, 8, and 24 h, respectively. H - J The expression of ATG5 and LC3B-II/I was detected by western blotting. Data were displayed as mean ± SEM . n = 3- 6. * P < 0.05, ** P < 0.01 vs 0 h group

Article Snippet: The mouse Sertoli cell line TM4 (GNM41) was obtained from Procell Life Science & Technology(Li et al. ).

Techniques: Injection, Transmission Assay, Electron Microscopy, Expressing, Western Blot

Journal: Drug and chemical toxicology

Article Title: Cytotoxicity of methamphetamine exposure on Sertoli cells: a pilot study with implications for male infertility.

doi: 10.1080/01480545.2025.2471383

Figure Lengend Snippet: Figure 1. The schematic outlines the experimental design to investigate the effect of acute exposure to Meth (24 hours) on cultures of Sertoli TM4 cells. In parallel, in the chronic study, we investigated the effects of daily exposure to Meth for 96 hours.

Article Snippet: the study was designed to investigate the Meth (sigma-aldrich, cat no. M8750, cape town, south africa) effect on an immortalized mouse sertoli cell line tM4 (atcc® cRl-1715) as this cell line is deemed useful as a model for testing male reproductive toxicity (Zheng et al., 2018). the tM4 cells were exposed for 24 hours indicative of the acute study and in the chronic study, tM4 cells were exposed daily for 96 hours (Figure 1). the rationale for treating cell cultures of tM4 cells for 24 hours was to mimic Meth exposure for the recreational user, who typically would only be exposed to Meth for a period of 24 hours. after 24 hours, Meth would be removed from the cultures, but we continued to monitor these cultures for 96 hours. the chronic study attempts to mimic the Meth addict who requires daily doses of Meth. in these experiments, the tM4 cell cultures were treated with Meth daily for 96 hours. in both the acute and chronic studies, mitochondrial function was assessed (Xtt assay) as well as to monitor permeability across monolayers of confluent tM4 sertoli cells using the transepithelial electrical resistance (teeR) technique. all experiments were carried out in triplicate at a minimum (n = 3) and duplicated to ensure repeatability.

Techniques:

Journal: Drug and chemical toxicology

Article Title: Cytotoxicity of methamphetamine exposure on Sertoli cells: a pilot study with implications for male infertility.

doi: 10.1080/01480545.2025.2471383

Figure Lengend Snippet: Figure 2. The effect of acute Meth exposure (24 hours) (A) and chronic Meth exposure (96 hours) (B) on the viability (%) of TM4 Sertoli cells over the experimental period of 24–96 hours using the trypan blue exclusion assay. Results were displayed as mean ± SEM (n = 3). *Statistical significance (p < 0.05) between experi mental samples as compared to controls by using ANOVA.

Article Snippet: the study was designed to investigate the Meth (sigma-aldrich, cat no. M8750, cape town, south africa) effect on an immortalized mouse sertoli cell line tM4 (atcc® cRl-1715) as this cell line is deemed useful as a model for testing male reproductive toxicity (Zheng et al., 2018). the tM4 cells were exposed for 24 hours indicative of the acute study and in the chronic study, tM4 cells were exposed daily for 96 hours (Figure 1). the rationale for treating cell cultures of tM4 cells for 24 hours was to mimic Meth exposure for the recreational user, who typically would only be exposed to Meth for a period of 24 hours. after 24 hours, Meth would be removed from the cultures, but we continued to monitor these cultures for 96 hours. the chronic study attempts to mimic the Meth addict who requires daily doses of Meth. in these experiments, the tM4 cell cultures were treated with Meth daily for 96 hours. in both the acute and chronic studies, mitochondrial function was assessed (Xtt assay) as well as to monitor permeability across monolayers of confluent tM4 sertoli cells using the transepithelial electrical resistance (teeR) technique. all experiments were carried out in triplicate at a minimum (n = 3) and duplicated to ensure repeatability.

Techniques: Trypan Blue Exclusion Assay

Journal: Drug and chemical toxicology

Article Title: Cytotoxicity of methamphetamine exposure on Sertoli cells: a pilot study with implications for male infertility.

doi: 10.1080/01480545.2025.2471383

Figure Lengend Snippet: Figure 3. The effect of daily Meth exposure on levels of dead cells (chronic study). From 48 to 96 hours, Meth tended to increase the number of dead cells compared to control TM4 cell cultures. Results were displayed as mean ± SEM (n = 3). *Statistical significance (p < 0.05) between experimental samples as compared to controls by using ANOVA.

Article Snippet: the study was designed to investigate the Meth (sigma-aldrich, cat no. M8750, cape town, south africa) effect on an immortalized mouse sertoli cell line tM4 (atcc® cRl-1715) as this cell line is deemed useful as a model for testing male reproductive toxicity (Zheng et al., 2018). the tM4 cells were exposed for 24 hours indicative of the acute study and in the chronic study, tM4 cells were exposed daily for 96 hours (Figure 1). the rationale for treating cell cultures of tM4 cells for 24 hours was to mimic Meth exposure for the recreational user, who typically would only be exposed to Meth for a period of 24 hours. after 24 hours, Meth would be removed from the cultures, but we continued to monitor these cultures for 96 hours. the chronic study attempts to mimic the Meth addict who requires daily doses of Meth. in these experiments, the tM4 cell cultures were treated with Meth daily for 96 hours. in both the acute and chronic studies, mitochondrial function was assessed (Xtt assay) as well as to monitor permeability across monolayers of confluent tM4 sertoli cells using the transepithelial electrical resistance (teeR) technique. all experiments were carried out in triplicate at a minimum (n = 3) and duplicated to ensure repeatability.

Techniques: Control

Journal: Drug and chemical toxicology

Article Title: Cytotoxicity of methamphetamine exposure on Sertoli cells: a pilot study with implications for male infertility.

doi: 10.1080/01480545.2025.2471383

Figure Lengend Snippet: Figure 4. The effect of acute Meth exposure (24 hours) (A) and chronic Meth exposure (96 hours) (B) on the proliferation of TM4 cells over the 24–96 hours exper imental period. *A statistically significant difference at 72 hours between experimental groups 0.1 µM, 1 µM, and controls, where the number of live cells in groups 10, 20, and 100 µM was significantly decreased (p < 0.05) (A, B). #In the chronic study, cells exposed for 96 hours to 0.1, 1, and 10 µM Meth were statistically no different from controls. However, exposure to 20 and 100 µM Meth significantly decreased (p < 0.05) the number of live cells compared to controls at 96 hours (Figure 4B). Data were displayed as mean ± SEM (n = 3).

Article Snippet: the study was designed to investigate the Meth (sigma-aldrich, cat no. M8750, cape town, south africa) effect on an immortalized mouse sertoli cell line tM4 (atcc® cRl-1715) as this cell line is deemed useful as a model for testing male reproductive toxicity (Zheng et al., 2018). the tM4 cells were exposed for 24 hours indicative of the acute study and in the chronic study, tM4 cells were exposed daily for 96 hours (Figure 1). the rationale for treating cell cultures of tM4 cells for 24 hours was to mimic Meth exposure for the recreational user, who typically would only be exposed to Meth for a period of 24 hours. after 24 hours, Meth would be removed from the cultures, but we continued to monitor these cultures for 96 hours. the chronic study attempts to mimic the Meth addict who requires daily doses of Meth. in these experiments, the tM4 cell cultures were treated with Meth daily for 96 hours. in both the acute and chronic studies, mitochondrial function was assessed (Xtt assay) as well as to monitor permeability across monolayers of confluent tM4 sertoli cells using the transepithelial electrical resistance (teeR) technique. all experiments were carried out in triplicate at a minimum (n = 3) and duplicated to ensure repeatability.

Techniques:

Journal: Drug and chemical toxicology

Article Title: Cytotoxicity of methamphetamine exposure on Sertoli cells: a pilot study with implications for male infertility.

doi: 10.1080/01480545.2025.2471383

Figure Lengend Snippet: Figure 6. The effect of acute Meth exposure (24 hours) (A) and chronic Meth exposure (96 hours) (B) on the mitochondrial activity (%) of the TM4 Sertoli cells in comparison to controls (not exposed to Meth) at selected time intervals. *The statically significant differences between experimental samples compared to the control at (p < 0.05), and #the significant differences between the treatment groups (p = 0.05). Data were represented as mean ± SEM (n = 5) by using ANOVA.

Article Snippet: the study was designed to investigate the Meth (sigma-aldrich, cat no. M8750, cape town, south africa) effect on an immortalized mouse sertoli cell line tM4 (atcc® cRl-1715) as this cell line is deemed useful as a model for testing male reproductive toxicity (Zheng et al., 2018). the tM4 cells were exposed for 24 hours indicative of the acute study and in the chronic study, tM4 cells were exposed daily for 96 hours (Figure 1). the rationale for treating cell cultures of tM4 cells for 24 hours was to mimic Meth exposure for the recreational user, who typically would only be exposed to Meth for a period of 24 hours. after 24 hours, Meth would be removed from the cultures, but we continued to monitor these cultures for 96 hours. the chronic study attempts to mimic the Meth addict who requires daily doses of Meth. in these experiments, the tM4 cell cultures were treated with Meth daily for 96 hours. in both the acute and chronic studies, mitochondrial function was assessed (Xtt assay) as well as to monitor permeability across monolayers of confluent tM4 sertoli cells using the transepithelial electrical resistance (teeR) technique. all experiments were carried out in triplicate at a minimum (n = 3) and duplicated to ensure repeatability.

Techniques: Activity Assay, Comparison, Control

Journal: Drug and chemical toxicology

Article Title: Cytotoxicity of methamphetamine exposure on Sertoli cells: a pilot study with implications for male infertility.

doi: 10.1080/01480545.2025.2471383

Figure Lengend Snippet: Figure 7. The effect of acute Meth exposure (A) and chronic Meth exposure (B) on TEER across TM4 Sertoli cell monolayers compared to controls at selective time intervals. Data are presented as mean ± SEM (n = 3). *Statistically significant differences in the TEER over time between experimental samples compared to the controls. Statistical significance was designated at p < 0.05 by using ANOVA.

Article Snippet: the study was designed to investigate the Meth (sigma-aldrich, cat no. M8750, cape town, south africa) effect on an immortalized mouse sertoli cell line tM4 (atcc® cRl-1715) as this cell line is deemed useful as a model for testing male reproductive toxicity (Zheng et al., 2018). the tM4 cells were exposed for 24 hours indicative of the acute study and in the chronic study, tM4 cells were exposed daily for 96 hours (Figure 1). the rationale for treating cell cultures of tM4 cells for 24 hours was to mimic Meth exposure for the recreational user, who typically would only be exposed to Meth for a period of 24 hours. after 24 hours, Meth would be removed from the cultures, but we continued to monitor these cultures for 96 hours. the chronic study attempts to mimic the Meth addict who requires daily doses of Meth. in these experiments, the tM4 cell cultures were treated with Meth daily for 96 hours. in both the acute and chronic studies, mitochondrial function was assessed (Xtt assay) as well as to monitor permeability across monolayers of confluent tM4 sertoli cells using the transepithelial electrical resistance (teeR) technique. all experiments were carried out in triplicate at a minimum (n = 3) and duplicated to ensure repeatability.

Techniques: